Abstract
The effect of mixed tumor-lymphocyte culture (MTLC) on the induction of lymphokine activated killer (LAK) cells was described. The LAK cells were generated from the PBLs of cancer patients by incubation in medium with rIL-2 (1000u/ml, Sionogi Pharmac. Co.) during several days. At the start of the LAK induction, the PBLs were stimulated with autologous tumor cells which were already treated with Mitomycin C. MTLC elevated the killer activity of the LAK cells significantly, compared with that of non-stimulated LAK cells. We also recognized the elevation of killer activity in the LAK cells stimulated with allogeneic tumor cells, accompanied with several differences among the samples. We discussed the effective procedures of MTLC.
