Abstract
Most non-hemolytic transfusion reactions (NHTR) following transfusion of authentic platelet concentrates (PC) into patients were allergic symptoms such as rash, itching and urticaria. To prevent these adverse effects, plasma of PC was removed by centrifugation, and then peletted platelets were resuspended in either saline solution including ACD-A solution or in modified Seto solution (MSS), which includes the acetate Ringer solution with glucose, sodium hydrogen carbonate, ACD-A, and water for injection. PH of MSS was 6.62. We compared aggregation responses to ADP plus collagen of washed platelets, which had been resuspended with continuous agitation immediately or 30min after the addition of the MSS additive solution. The aggregation responses of 72-hr-old platelets were maintained when plasma-depleted platelets were resuspended with MSS after the 30-min rest period. The average CCI of transfused platelets in MSS was 7576 at one day after.
Mean incidence rates of adverse reactions by transfusion of washed PC were dramatically decreased from 50.4% to 2.2% in 71 patients compared to rates with the authentic PC. The present data indicate that platelets washed with MSS are a useful alternative in decreasing allergic NHTR in patients.
