INFLUENCES OF LEUKOCYTES ON THE PRESERVATION OF BLOOD CELLS

Abstract

For the purpose of studying the influences of leukocytes on the preservation of blood cells, the author prepared blood samples containing more or less leukocytes from blood collected in ACD solution, by separating leukocytes as buffy coat and by removing leukocytes with serum albumin solution (human), the specific gravity having been adjusted to lie between those of erythrocytes and leukocytes, and observed the changes of the blood during storage at 4-6°C.
1) Influence of leukocytes during storage was not noticeable on the changes of hematocrit value, agglutinability of erythrocytes, amino-nitrogen contents of plasma, fibrinolysis and amino-acids in plasma as observed by paper-chromatography.
2) It was observed that leukocytes accelerated the decrease in the erythrocyte sedimentation rate, the decline of blood sugar level and the increase of osmotic fragility of erythrocytes or of free hemoglobin content in plasma during storage.