The purpose of the present study were to develop a scale to measure individual differences in health-related life skills in adolescents, and to examine the relationship between evaluation of the health-related life skills and sport experience. The scale was based on the framework of health that WHO (1994) was defined, which includes 3 aspects: physical, psychological, and social. In study 1, we analyzed 1755 adolescents (846 junior high school students, 557 high school students, and 352 university students). A factor analysis for each of the 3 scales was conducted to find the factor structure. Consequently, 6 factors were extracted: physical skills (physical activity skills and health maintenance skills), psychological skills (goal execution skills and coping with stress skills), and social skills (group activity skills and interpersonal skills). The results also showed that the scale was moderately reliable and valid for measuring health-related life skills in adolescents. In study 2, another survey was carried out on 304 high school students who had experienced athletic involvement and 215 students who did not have experienced any athletic involvement. From the results, it was clarified that students who had experienced athletic involvement acquired more health-related life skills than students who did not have experienced any athletic involvement. These results suggested that sport experience has influenced health-related life skills.