Experimental studies on the Allergic Granulocytopenia

Abstract

Rabbits, sensitized with human sera, were treated repeatedly with injections of the antigen to cause an allergic granulocytopenia.
Granulocytes almost disappeared from the peripheral blood of the rabbits 10-60 minutes after the shock injection, andthen,1-2 hours after the injection, a remarkable increase of the cells was observed, accompanied with the shift to the left of nuclei, appearance of degenerated cells and nucleous shadows. The granulocyte count decreased again in 24-48 hours and recovered to the normal level in 5-6 days.
By repeated shock injections, the granulocyte count kept a low level, which was not recovered to the normal at least 2 weeks after the last injection. In the bone marrow, nucleated cells, especially immature granulocytes, were remarkably decreased.
On the contrary, unsensitized animals showed neither shock syndrome, nor granulocytopenia after 3 times injections of human sera.
Red cell count, hemoglobin content and thrombocyte count also gradually came down accompanied with the allergic granulocytopenia. Increase of reticulocytes, appearance of erythroblasts in the peripheral blood and increase of polychromatic normoblasts in the bone marrow were also observed.