3'-Methy 1-4-dimethyl - aminoazobenzene (3'-MeDAB)肝ガン発生過程に見られるラツテ肝実質細胞内諸酵素活性変動の超微細胞化学的研究

I.酸性ホスフアターゼ(ACPase)とリゾゾーム

抄録

The activity of acid phosphatase (ACPase) in normal rat liver and in the rat liver during the course of carcinogenesis induced by 3'-methyl-4-dimethyl-diaminoazobenzene (3'-MeDAB) was studied ultracytochemically up to 34 weeks. From 24 weeks the hepato-cellular hepatomas could be obtained.
The ACPase activity was positive in lysosomes including cytolysomes in the normal hepatic parenchymal cells. The enzymatic activity in hepatic cells in the early and intermediate stages of carcinogenesis showed some decrease which was reflected in number and size of lysosomes. However, the activity in hepatic cells revealed increase in late stages of carcinogenesis as well as in hepatocellular hepatoma cells. The probable role of the ACPase activity in the azc-dye carcinogenesis was briefly discussed.
Supported by grants from the Anna Fuller Fund, the Jane Coffin Childs Memorial Fund for Medical Research (Project # 196) and the Japanese Government (Gann 94157) administered by Prof. K. Ogawa.