抄録
Fructose 1,6-diphosphate (FDP) or fructose 1-phosphate (FIP) is used as substrate in determining aldolase (ALD) activity. An FDP/FIP activity ratio, toward the substrates of FDP and FIP is about 1 for ALD B, and about 50 for ALD A. The malignant tissue shows an increase in ALD A resulting in an increase of FDP/FIP activity, ratio in the serum.
Presently determination of FDP/FIP was made in digestive diseases cases' serum, tissue and the serum of rats with experimental hepatic disturbances. The ALD zymogram was made of the organ tissues of human subjects and animals.
1) Serum FDP/FIP activity ratio was 2.7 in healthy subjects,9.3 in gastric cancer cases and 10.4 in hepatic cancer cases. It was low, being almost equal to normal, in cases of benign digestive diseases.
2) Tissue FDP/FIP acti vity ratio proved to be 45.5 for the skeletal muscle,10.4 for the brain and 1.27 for the liver. It was 13.7 in the cancerous region,2.0 in the grossly normal region of liver cancer and 1.7 in the liver tissue of cancer patients without liver metastasis. It was markedly high in the region with a hepatic cancer infiltration.
3) FDP/FIP activity ratio in serum of rats with experimental hepatic disturbanc es. In cases of CC14 hepatic disturbances or common bile duct ligation. FDP/FIP was decreased, but in the regenerated liver the ratio which had decreased post-operatively showed a gradual, daily increase regaining the previous ratio in five days.
4) The ALD zymogram. Liver: In human subjects there were the A band and B band; a hybrid was observed near to the B band. In rats, only the B band was observed. Skeletal muscle: Human subjects and rats showed the A band near to the origin in the anode side. Human hepatic cancer: In the cancerous region only the A band was observed. DAB hepatoma: The B band became light and the A band appeared. Serum: On electrophoresis of acute hepatitis cases' sera a band was found at the B region. Determination of an FDP/FIP activity of serum and tissue A LD seems valuable in differentially diagnosing between the malignant and benign nature of digestive disease.
