抄録
Experiments were designed in order to study autoradiographically the effects of anticancer drugs on DNA and RNA synthesis in YS cells cultivated on the CAM of embryonated eggs. On the other hand, the blood levels of anti-cancer drugs in the chicken embryos were estimated by utilizing their anti-microbial activities (“Band culture method”, OKUBO).
Results
1) Cyclophosphamide inhibited markedly and mitomycin C moderately the thymidine incorporation by YS cells, while cytosine arabinoside did not affect it. As to the uridine incorporation by YS cells, daunomycin and 5-fluorouracil showed marked inhibition, while β -methasone and chromomycin A3 showed no effect.
2) The peak levels of each anti-cancer drug in the serum of 13-14 days old chicken embryos after yolk sack injection of 100mcg mitomycin C,5mg 5-fluorouracil,800mcg bleomycin, or 1mg daunomycin were 0.8mcg/ml,160mcg/ml,11 mcg/ml, and 0.45mcg/ml respectively; while the administration of 50mcg chromomycin A3 yielded no detectable serum level (< 0.1mcg/ml). The kinetics of these anti-cancer drugs in the chicken embryos was discussed.
