2018 Volume 64 Issue Suppl.1 Pages 107-113
Objective: To examine the effects of NADPH oxidase (NOX) and xanthine oxidase (XO) inhibition on the activation of AMPK signaling after exercise in skeletal muscle.
Methods: Adult male C57BL/6J mice were assigned to four weight-matched groups (n=8/group): 1) sedentary control (CON), 2) exercise (Ex), 3) exercise+NOX inhibitor (Ex+Apo), and 4) exercise+XO inhibitor (Ex+Allo). Animals in the Ex, Ex+Apo, and Ex+Allo groups were subjected to running on a treadmill at 15 m/min for 60 min. Animals in the Ex+Apo or Ex+Allo groups were intraperitoneally injected with a NOX inhibitor (apocynin, 20 mg/kg) or XO inhibitor (allopurinol, 20 mg/kg) 30 min before exercise. After exercise, the plasma levels of reactive oxygen metabolites (d-ROMs) were measured as oxidative stress marker, and the phosphorylation levels of AMPKα and ACC in the gastrocnemius muscle were determined by Western blot analysis.
Results: The d-ROMs level was significantly increased in the Ex group compared with the CON group, while the increase in d-ROMs was suppressed by NOX and XO inhibition. In addition, the phosphorylation levels of AMPKα at Thr172 and ACC at Ser79 in the Ex group was significantly increased compared with that in the CON group (p<0.05), whereas the phosphorylation levels in the Ex+Apo and Ex+Allo groups did not increase.
Conclusions: NOX and XO inhibition repressed the expected increase in oxidative stress following exercise and attenuated the activation of AMPK singling after exercise in skeletal muscle.
