Juntendo Medical Journal
Online ISSN : 2188-2126
Print ISSN : 2187-9737
ISSN-L : 2187-9737
Original Articles
Detection of Arcobacter Species in Human Stool Samples by Culture and Real-time PCR
YUKO YAMAUCHIYUKI UEHARASÉBASTIEN BOUTINNORIO YAMAMOTOKYOKO KUWAHARA-ARAITERUO KIRIKAEKEIICHI HIRAMATSUSTEFAN ZIMMERMANN
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JOURNALS OPEN ACCESS

2020 Volume 66 Issue 5 Pages 431-438

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Abstract

Objective: The clinical significance of Arcobacter species has not been established due to a lack of suitable detection methods.

Material and Methods: A total of 1,650 stool samples submitted to the Clinical Laboratory of Heidelberg University Hospital were inoculated onto agar plates selective for Campylobacter species isolation and incubated at 37℃.

Results: Four (0.24%) of the samples were positive for Arcobacter butzleri isolates. Genus-specific primers for real-time PCR were designed to identify Arcobacter species. Of the 1,650 stool samples tested, twelve (0.73%), including the four culture-positive samples, were positive for Arcobacter species by real-time PCR. The sensitivity of real-time PCR was 10 4 CFU g -1 stool, 50 CFU reaction -1 using a stool sample.

Conclusions: Although the sensitivity of real-time PCR was relatively low compared with other PCR methods, the present method detected a broad range of Arcobacter species. The combination of the stool culture using agar selective for Campylobacter species and real-time PCR for Arcobacter species may be clinically useful for the diagnosis and epidemiology of Arcobacter species infections.

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© 2020 The Juntendo Medical Society. This is an open access article distributed under the terms of Creative Commons Attribution License (CC BY), which permits unrestricted use, distribution, and reproduction in any medium, provided the original source is properly credited.

This article is licensed under a Creative Commons [Attribution 4.0 International] license.
https://creativecommons.org/licenses/by/4.0/
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