哺乳動物卵子研究会誌
Online ISSN : 1884-6505
Print ISSN : 0289-5439
ISSN-L : 0289-5439
ラットとマウスにおける異属間集合キメラ胚の作成
高橋 文明藤代 克彦高橋 寿太郎安田 泰久
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1986 年 3 巻 2 号 p. 73-77

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An attempt for obtaining xenogeneic blastocysts (Rat↔Mouse) was undertaken by aggregation method. As a culture medium, Dulbecco's modified eagle medium with 0.3% bovine serum albumin (pH 7.4) was used for the production of xenogeneic chimeric embryos in this experiment.
When the late eight-cell stage rat and mouse embryos were cultured immediately after flushing from the uterus, 88.2%(30/34) of rat embryos and 95.0%(19/20) of mouse embryos developed into expanded blastocyst after 48 hours in culture. When rat and mouse embryos were removed their zona pellucidae with 0.3% pronase before culture, 87.0%(20/23) of rat embryos and 95.7%(22/23) of mouse embryos developed into expanded blastocyst after 48 hours in culture. On the other hand, 87.0%(40/44) of allogenic chimeric rat embryos (Rat↔Rat) and 90.5%(38/42) of allogenic chimeric mouse embryos (Mouse↔Mouse) developed into single, integrated expanded blastocyst after 48 hours in culture respectively. Furthermore, xenogeneic chimeras betwen rat and mouse were produced by aggregating embryos at the late eight-cell stage in this experiment. Out of 41 aggregated embryos from rat and mouse, 34 (82.9%) formed single, integrated expanded blastocyst after 48 hours in culture.
These results showed that Dulbecco's modified eagle medium was available for the production of xenogeneic chimeras between rat and mouse.

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