抄録
In order to survey the relationships between metabolitic activity and develpmental ability of bovine embryo derived from in vitro fertilization, we modified an enzymatic cycling method in the present study. We measured lactate dehydrogenase (LDH) in preimplantation stages of single bovine embryo derived from IVF to examine the effect of LDH activity on a subsequent development in vitro. LDH activity of bovine embryos varied with the cleaving stages and embryos quality (Table 1, 2). LDH activity in the degenerated embryos was the highest as compared with that in normal embryos. It is suggested that the release of LDH by dying or degenerating embryos might offer an alternative for determining 2 the viability of embryos. Thus, the LDH activity proved to be a practical method fordistinguishing between nonviable and viable embryos.
