抄録
We have attempted an establishment of ES cells from bovine blastocysts fertilized in vitro co-cultured with fibroblast feeder cells of murine or bovine embryos. In this report, we have shown bovine ES-like cells from co-culture used murine fibroblasts as feeder layer. The ES-like cells were compared morphologically with trophoblast whose features are like ES cells. Furthermore, when the ES-like cells were exposed to 10-5M alltrans-retinoic acid, these cells differentiated into neuron-like cells. And, it was observed that the growth of ICMs of blastocysts used were slow, some of them hardly grew. These results may suggest that the growth of ICMs is influenced by differences of genome of each embryo, and also indicat that the property of ICMs is different between murine and bovin.
