抄録
A [Fe] hydrogenase gene (hyd) was cloned from Clostridium paraputrificum in Escherichia coli using a probe of a conserved DNA sequence of clostridia hydrogenase genes amplified by PCR. The hyd consists of an open reading frame of 1, 749-bp, encoding 582 amino acids with a MW of 64, 560. The cloned hyd was ligated into a shuttle vector pJIR751 between E. coli and C. paraputrificum and was sequenced and expressed in C. paraputrificum. The hydrogen gas productivity of the transformant was increased up to 1.7-fold. Since hydrogenase encoded by hyd is the terminal enzyme of the sequential metabolism for hydrogen evolution, fortification of the enzyme in the cell might accelerate hydrogen release.
