2020 年 66 巻 Supplement 号 p. S336-S342
In this study, the protective role of turmeric on genotoxic effects of Bisphenol-A exposure in Wistar rats by in vivo experiment were investigated. Bisphenol-A is a known endocrine disruptor and suspected carcinogen, that comes diet through plastics for food packaging and food processing. In this study, rats were divided into three groups of twelve animals each and were administered with Bisphenol-A by oral gavage with levels of 0, 50 and, 100 μg. Half of the animals in each group were fed with feed which contained 3% turmeric (wt/wt), for a period of 4 wk, while the rest of the rats received the same diet treatment without the addition of turmeric. At the end of the experiment, all rats were terminated and the internal organs such as liver, kidney, femurs were collected and analyzed. Mean and SD values were compared by one-way ANOVA and Kruskal-Wallis-Wilcoxon test, the formation of micronuclei was compared using Mann-Whitney U-test. Significant decrease in serum malondialdehyde and urinary 8-hydroxy-2′-deoxyguanosine levels were observed in Bisphenol-A+turmeric groups as compared to Bisphenol-A groups. Bisphenol-A groups exhibited significantly higher mean levels of DNA damage in liver and kidney as compared to the untreated control group. Bisphenol-A group showed significant increase in the formation of micronuclei which was approximately threefold higher as compared to the control group. A significant decrease in DNA migration was observed in Bisphenol-A+turmeric fed groups in liver and kidney. Turmeric feeding significantly inhibited the micronuclei formation induced by Bisphenol-A. The study results indicate that turmeric can protect against Bisphenol-A induced genotoxicity in rats.
