抄録
A fluorometric determination for PIN was described and the effects of various conditions were reported. Under the conditions of the determination, PIN is completely recovered through the entire procedure, PAM, PAL and lactone are practically without effect. PIC itself can interfere remarkably but in the preliminary extraction procedure it is completely converted into its lactone, thus becoming ineffective. For extraction and hydrolysis of tissue PIN, the preliminary extraction at pH 4.5 at 80° for 15 minutes and the successive hydrolysis of the filtered extract in 0.6N H2SO4 at 130° for 1 hour is most suitable in cases of both plant and animal tissues. For neutralizing the acid extract, the partial neutralization by barium hydroxide for removing sulfate ions is beneficial for promoting recovery.
