Abstract
The possibility of the formation of protein-thiamine mixed disulfide by the reaction of thiol type thiamine and the SS-groups of natural protein was studied using bovine serum albumin and the following results were obtained.
1. Under aerobic condition, much protein-bound thiamine was produced increasing with the rise of reaction time and pH.
2. Bound thiamine was also formed when γ-globulin, which contains as much SS-groups as bovine serum albumin, was used, but it was not the case with the bovine serum albumin, whose SS-groups had been partially reduced.
3. Under anaerobic condition, however, only a small amount of bound thiamine was produced. When the reaction mixture was subjected to Sephadex column chromatography, a small part of the SS-group of the protein was found to have been reduced.
4. From these results it is assumed that the production of much bound thiamine under aerobic condition is due to the thiamine disulfide produced by the action of dissolved oxygen and that the thiol-type thiamine per se can react only partially with the SS-group of bovine serum albumin.
