1. Toxicity of vitamin D2 sulfate in saline solution in mice was tested by intraperitoneal injection. The lethal dose, LD50, in mice, weighing about 20g, was about 2, 5000, 000I.U./kg.
2. Antirachitic potency of vitamin D2 sulfate was tested by prophylactic method and it was found that the compound had about the same effect as vitamin D2.
3. By administering 5, 000I.U. of the compound or 10I.U. of vitamin D2 per head per os daily, gained the body weight till 15 days, whereas those receiving 5, 000I.U. of vitamin D2 per head per os daily resulted in the failure to gain weight after 10 days.
