抄録
1. Coenzyme B12 content of liver and kidney was measured by the coenzyme B12 activity in tne intramolecular oxidation-reduction reaction of α-glycol. According to Abeles the apoenzyme was prepared from Aerobacter aerogenes. Following Baker's method, coenzyme B12 was extracted from the liver and kidneys of human and some animals.
2. The content of coenzyme B12 was measured in rats, rabbits, guinea pigs and human subjects. The average value of coenzyme B12 content in normal rat liver was 0.68±0.35μg/g wet weight and that of kidney was 1.88±1.08. The average value of rabbit liver was 1.18±0.25 and that of kidney 1.65±0.61, the values larger than those of rats and guinea pigs. The average value of coenzyme B12 content of human liver without liver injury was 1.23±0.31. Coenzyme B12 content of the liver and kidneys of the rat maintained on a vitamin B12-deficient diet was smaller than those of the normal rat.
Coenzyme B12 content of the liver and kidney of the rat saturated with 100mμg of vitamin B12 did not differ so much from those of the normal rat. In the rats having acute liver injury due to carbon tetrachloride, the coenzyme B12 in the liver and kidneys decreased remarkably from 6 to 9 hours after liver damage, and a sign of recovery of the coenzyme content was recognized 15 hours after damage. Thereafter, the coenzyme content approached to that of the normal rat.
