Studies on Human Gastric Intrinsic Factor

II. In Vitro Assay of Human Gastric Juice and Purified Human Intrinsic Factor for Intrinsic Factor Activity

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1. Everted sacs of guinea pig intestine were useful to assay the intrinsic factor activity of the purified human gastric intrinsic factor when pernicious anemia patients or totally gastrectomized patients were not available.
2. The author's purified gastric intrinsic factor was found quite active and the activity was destroyed by heating and peptic digestion.
3. Gastric juice from three patients having histamine-fast anacidity without pernicious anemia were confirmed to possess intrinsic factor (IF) by this in vitro assay method.
4. Gastric juice from three patients with non-Addisonian megaloblastic anemia gave greater uptake of ⁵⁷Co-B₁₂ by the sac than that in controls where the sac was incubated with ⁵⁷Co-B₁₂ alone, showing the presence of intrinsic factor in the gastric juice.
5. The gastric juice from pernicious anemia patients showed a rather suppressive effect on ⁵⁷Co-B₁₂ uptake by the sac as compared with the uptake in controls. The presence of the substance inhibiting B₁₂ uptake in the gastric juice of pernicious anemia patients was discussed.