抄録
The amount of P32 in FMN used as a substrate and that in FAD obtained enzymatically were proved to be equal. In other words, the phosphoric acid of FMN was incorporated in toto in FAD, according to the formula previously indicated. The determination of pyrophosphoric acid was fairly difficult; Schrecker and Kornberg (6), in the biosynthesis of diphosphopyridine nucleotide, have demonstrated the formation of the nucleotide and pyrophosphoric acid by directly determining both substances from nicotinamide mononucleotide and ATP.
As for FAD, it has not been possible so far, to prove directly the reaction mechanism by determining pyrophosphoric acid. With the use of P32, it was possible to contribute to an analysis of this reaction mechanism.
