THE ISOLATION OF ANILINOTHIAMINE FORMED FROM THIAMINE AND ANILINE BY BACILLUS ANEURINOLYTICUS KIMURA ET AOYAMA

Abstract

The production of anilinothiamine by Bacillus aneurinolyticus Kimura et Aoyama in the presence of both thiamine and aniline was confirmed by isolating the crystals and by comparing the elementary analysis and physical constants with those of the synthetic compound. The yield of the isolated anilinothiamine was not clear, because the pyrimidine moiety which is produced simultaneously absorbs remarkably at 245mμ, thus making the separate determination of anilinothiamine impossible. For isolating the crystals the application of ion exchange resin column chromatography was proved to be efficicient.