ON THE DETERMINATION OF PLASMA VITAMIN A USING GLYCEROL DICHLOROHYDRIN

Abstract

As the glycerol dichlorohydrin methods thus far reported did not give the proportional relationship between the vitamin A concentrations and optical densities with the Beckman Spectrophotometer, the condition was reexamined and an appropriate procedure was described. Cholesterol was proved to be practically without effect on the vitamin determination. Carr-Price method showed a fair proportionality between concentrations and optical densities.
The plasma vitamin A levels of 6 healthy Japanese adults were determined both by GDH method and Carr-Price method and gave practically same values, i.e., 137±30 I.U. per 100ml.