抄録
In determining riboflavin in human urine the presence of non-riboflavin fluorescent substances disturb occasionally the fluorometry. They were shown to be anthranilic acid and its conjugate, glucuronide.
The conjugate was hydrolyzed into the free form which was identified as anthranilic acid by paper chromatography. The sugar produced was also identified as glucuronic acid by the same method.
For removing anthranilic acid conjugate, oxidation by permanganate and a preliminary chloroform extraction from alkaline solution after photolysis were found to be the most effective means.
