抄録
In order to prepare the mycelia of Er. ashbyii as a raw material for extracting FAD, some basal cultural conditions were examined. In these experiments, the FAD stored in the mycelia amounted to 60-120mg/l, and the highest yield obtained was 194mg/l, whereby a shaking culture was used with a medium containing 1% glucose, 1.5% peptone, and 0.1% CSL and incubated for 72hr. In general, with the rise in mycelial growth, the FAD production in the mycelia seems to be decreased.
Preparation of dry mycelia was tested by 3 methods including dehydration with dry acetone and drying by heat. Loss of FAD amounted to about 30% of the initial values. However, the FAD in dried mycelial preparations was stable at least for 6 months in a desiccator. The results of cultures in a 2-ton fermentor were also reported.
