CONTRIBUTION OF VITAMIN B₁₂ TO ACTIVATION OF APOENZYME FOR FORMING α-KETOGLUTARATE OXIDASE SYSTEM IN RATS

抄録

1. The apoenzyme activity of α-ketoglutarate oxidase system in the tissue of B₁₂-deficient rat studied in vitro under various addition of B₁₂, cytochrome, niacinamide, DPN, DPT, lipoic acid, CoA, ATP, FAD and glutathione. The decrease of the apoenzyme activity in B₁₂-deficient rats was always recognized in the uptake of O₂ by α-ketoglutarate oxidation system.
TABLE III Uptake of O₂ by α-Ketoglutarate Oxidation System in Washed Liver Homogenate of B₁₂-deficient Rats and of Rats Receiving B₁₂
2. The animals receiving 0.2μg of B₁₂ per os daily before experiments showed always an increased apoenzyme activity of α-ketoglutarate oxidase system.
3. The results seem to lead to the conclusion that vitamin B₁₂ contributes to the activation of the apoenzyme in α-ketoglutarate oxidase system in rats.