抄録
1. Male weaning rats, maintained on a low fat, vitamin E-free diet for several weeks were divided into three groups, termed respectively, vitamin E-deficient, control and vitamin E-excess.
2. As a daily dietary supplement 0.5mg α-tocopherol was added to the diet of each animal in the control group, and 100mg α-tocopherol was added to the diet of each in the E-excess group.
3. Glutamic-aspartic transaminase, glutamic dehydrogenase activities, and free amino acids and protein were determined in the liver tissue of all the experimental animals.
4. On a wet weight basis, excess vitamin E reduced only the free amino acids of liver tissue, while vitamin E deficiency increased only glutamic-aspartic transaminase activity.
5. On a cellular basis, excess vitamin E brought about a reduction in glutamic-aspartic transaminase activity, glutamic dehydrogenase activity, protein and free amino acids; on the same basis deprivation of vitamin E caused a significant increase in glutamic-aspartic transaminase activity only.
6. The significance of selecting a proper reference base is discussed.
