Abstract
1. The unadapted cells are not capable of adapting for ascorbic acid, even if they are in contact with ascorbid acid for a long time, but they can adapt rapidly in the presence of a small amount of glucose and of casein hydrolysate.
2. Ascoraic acid-adapted cells degrade araboascorbic acid and glucoascorbic acid besides AsA, but fail to degrade other reductones, e.g., reductic acid, dihydroxymaleic acid and 5-methyl-3, 4-dihydroxytetrone.
3. The optimal pH of the ascorbic acid-oxidase in the unadapted cells is 5.0 and the optimal substrate concentration is 800μM.
4. The ascorbic acid-oxidase is inactive for the reductones other than ascorbic acid i.e., araboascorbic acid, glucoascorbic acid, reductic acid, dihydroxymaleic acid and 5-methyl-3, 4-dihydroxytetrone.
