CHEMICAL STUDIES ON VITAMIN B₁₂ AND ITS RELATED COMPOUNDS

VI. PAPER-ELECTROPHORETIC EXAMINATION ON COBAMIDES IN PROPIONIBACTERIUM SHERMANII CELLS CULTIVATED IN THE PRESENCE OF SELECTED PRECURSORS

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An attempt was made to elucidate the natural forms of the cobamides synthesized in Propionibacterium shermanii grown in the culture medium containing both a cobinamide-like compound (X factor) and 5, 6-dimethylbenzimidazole as precursors. The cells were homogenized in alcohol and the extract was examined by paper electrophoresis, whereby at least 4 forms of cobamide, including Barker's coenzyme and a protein-bound form were observed.