FLUCTUATION OF THE NUCLEOTIDE POOLS OF FLAVINOGENIC AND NONFLAVINOGENIC STRAINS OF EREMOTHECIUM ASHBYII GROWN IN THE PRESENCE OF PURINES
1973 年 19 巻 1 号 p. 29-42
詳細
1973 年 19 巻 1 号 p. 29-42
Fluctuation of the nucleotide pools in a flavinogenic and a nonflavino-genic strains of Eremothecium ashbyii was followed by using column chromatography with an anion exchanger, and effects of purine bases on the pools were tested throughout the growth cycle.
1) Contents of the nucleotides in the flavinogenic strain were detected to be maximum at 1 day culture prior to the growth maximum (2 days culture), while in the nonflavinogenic strain the largest pools were ob-served after 4 days culture, corresponded to the growth maximum. After 1 day cultivation the pools in the former strain shrank rapidly with the culture time: this was remarkable for ATP pool.
2) It was found in the flavinogenic fungus that GMP pool decreased more slowly than AMP pool at the exponential stage of riboflavin for-mation.
3) Addition of purine bases into the culture media resulted in expantion of ATP pool and simultaneous shrink of AMP pool in the flavinogenic strain, although sum of the contents of adenosine nucleotides remained almost constant. However, such phenomenon was not observed in the nonflavinogenic strain.
4) Growth maximum of the nonflavinogenic fungus was obtained at 4 days culture, but riboflavin was synthesized with the maximum yield after 7 days cultivation.
