A METHOD FOR QUANTITATION OF VITAMIN B₂ BY USING AN ENZYME (PRODUCING VITAMIN B₂ ALDEHYDE) FROM SCHIZOPHYLLUM COMMUNE

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A method for enzymatic determinationn of riboflavin was developed by enzymatic reduction of 2, 6-dichlorophenolindophenol using a vitamin B₂-aldehyde-forming enzyme prepared from Schizophyllum commune. The enzymatic method enabled us to determine riboflavin in a mixture containing riboflavin, FMN and FAD without separation of riboflavin. Moreover, the total amount of flavins in the mixture was shown to be determined by the enzyme in the presence of phosphatase.