(III) Quantitative Estimation of Fluorine Contained in Hard Tissues

Abstract

In chronic fluorine poisoning, Osteosclerosis and mottled teeth are observed. In order to clarify the relation between such degeneration and the amount of fluorine contained in hard tissues, an experimental study was conducted in rabbits. An apparatus for automatic distillation of fluoride, best suited for quantitative estimation of fluorine in hard tissues, was prepared and the data was subjected to analysis. The process of disintegration and effect of interfering were clarified with the method of sodium fusion and of ion exchange resin. The result obtained were as follows: 1. By fixing the time of distillation to 40 minutes and the quantity of distilled liquid to 250ml, more than 96% will be recovered. 2. Disintegration with perchloric acid is perfect, and practically no effect of interfering ion will be noted. 3. In the cases of control, 248, 214 ppm fluorine was noted in the calcification of the femur and incisor. 4. Degeneration of a bone will be noted in the similar tissues, when more than 625 ppm of fluorines is deposited. 5. With more than 1973 ppm of fluorine deposition, mottled teeth will result. 6. Dental abrasion of incisor will be also cased with more than 572 ppm of fluorine deposition.