Conditioned Media from Human Dental Pulp Stem Cells Prevent Radiation-induced Skin Injury

Keita KAWARABAYASHI; Fumiya KANO; Noboru HASHIMOTO; Hideharu HIBI; Tsutomu IWAMOTO; Akihito YAMAMOTO

doi:10.20738/johb.33.1_1

Original Article

Conditioned Media from Human Dental Pulp Stem Cells Prevent Radiation-induced Skin Injury

Keita KAWARABAYASHI , Fumiya KANO, Noboru HASHIMOTO, Hideharu HIBI, Tsutomu IWAMOTO, Akihito YAMAMOTO

Author information

Keita KAWARABAYASHI [Japan] Corresponding author
Department of Tissue Regeneration, Institute of Biomedical Sciences, Tokushima University Graduate School [Japan]
Pediatric Dentistry, Institute of Biomedical Sciences, Tokushima University Graduate School [Japan]
Fumiya KANO [Japan]
Department of Tissue Regeneration, Institute of Biomedical Sciences, Tokushima University Graduate School [Japan]
Noboru HASHIMOTO [Japan]
Department of Tissue Regeneration, Institute of Biomedical Sciences, Tokushima University Graduate School [Japan]
Hideharu HIBI [Japan]
Department of Oral and Maxillofacial Surgery Nagoya University Graduate School of Medicine [Japan]
Tsutomu IWAMOTO [Japan]
Pediatric Dentistry, Institute of Biomedical Sciences, Tokushima University Graduate School [Japan]
Akihito YAMAMOTO [Japan]
Department of Tissue Regeneration, Institute of Biomedical Sciences, Tokushima University Graduate School [Japan]

Keywords: Human dental pulp stem cells, conditioned media, mesenchymal stem cells, Radiation-induced skin reactions, Cutaneous radiation syndrome

JOURNAL FREE ACCESS

2020 Volume 33 Issue 1 Pages 1-7

DOI https://doi.org/10.20738/johb.33.1_1

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Abstract

Radiation-induced skin ulceration is a frequent complication of radiotherapy for cancer treatment. Stem cells from human exfoliated deciduous teeth (SHEDs) can regenerate various tissues. In this study, we investigated the impact of SHED-conditioned medium (SHED-CM) on radiation-induced skin injury. Mouse necks were locally irradiated with a single dose of 15 Gy of radiation. A week after the irradiation, most of the wild-type mice generated ulcer surrounded by severe erythema. Intra-venous administration of SHED-CM effectively inhibited the ulcer formation. Histological examination revealed that SHED-CM treatment inhibited radiation-induced dermal thickness and epithelial hyperplasia. SHED-CM could be a useful treatment option for radiation-induced skin ulceration.

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