Radiation-induced skin ulceration is a frequent complication of radiotherapy for cancer treatment. Stem cells from human exfoliated deciduous teeth (SHEDs) can regenerate various tissues. In this study, we investigated the impact of SHED-conditioned medium (SHED-CM) on radiation-induced skin injury. Mouse necks were locally irradiated with a single dose of 15 Gy of radiation. A week after the irradiation, most of the wild-type mice generated ulcer surrounded by severe erythema. Intra-venous administration of SHED-CM effectively inhibited the ulcer formation. Histological examination revealed that SHED-CM treatment inhibited radiation-induced dermal thickness and epithelial hyperplasia. SHED-CM could be a useful treatment option for radiation-induced skin ulceration.

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Background: Metal allergy is caused by many factors, including cells, cytokines, chemokines, or the environment. Recent studies suggested semaphorin7A (Sema7A), expressed on activated T cells, is crucial in producing inflammation through α1β1 integrin on monocytes and macrophages. However, the role of Sema7A on keratinocytes in metal allergy is still unclear. In this study, we focused on keratinocytes since they are known as an important player for skin immunity, and analyzed the effect of Sema7A expressed on keratinocytes in the development of metal allergy.
Materials and Methods: Mouse keratinocyte line PAM2.12 cells were treated with NiCl₂ to analyze the expression of Sema7A. Ni allergy was induced in female C57BL/6J mice (6-8 weeks old) with or without Sema7A suppression to confirm if Sema7A is necessary in producing allergic reactions to NiCl₂.
Results: NiCl₂ enhanced the expression of Sema7A in a dose and time-dependent manner after 72 hours of stimulation. PAM2.12 produced TNF-α in response to NiCl₂, and this secretion was reduced by Sema7A inhibition. In a mouse model, ear thickness, at 48 hours after NiCl₂ injection, significantly decreased by Sema7A siRNA administration.
Conclusions: Sema7A is essential in producing an allergic reaction to NiCl₂, especially during the effector phase. Since the interaction between Sema7A and α1β1 integrin enhances inflammation in many skin diseases, this interaction may also have possibilities to be a therapeutic target for metal allergy.

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The term, periodontal medicine is used to describe the multitude of systemic diseases which are regarded to link periodontal disease. The concept of periodontal medicine has been widely accepted today, however, the molecular mechanisms which periodontal diseases impact general health in whole body are not elucidated in detail. Extracellular vesicles (EVs) and outer membrane vesicles (OMVs) are the nano-sized particles released from mammalian cells and bacterial cells resectively, which influence the health and various disease by transporting biological factors to the neighbor and distant cells. In this review, we will discuss whether EVs and OMVs produced in periodontal diseases could be implicated in periodontal medicine.

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It is predicted that the population of Tokushima Prefecture will decrease to 550,000 by 2040, and 17 municipalities will disappear. For that reason, the community-based integrated care system (integrated care) must be further developed. The purpose of integrated care is the realization of "community development which you can continue to live as you are in the area where you are accustomed to living". To achieve this, not only medical and care, but also the utilization of new human resources and resources has been demanded. In Japan, based on the Sustainable Development Goals (SDGs), relevant laws and systems will be revised to achieve the agenda set forth by 2030, and it has been strongly suggested that it is necessary to participate the welfare area (Long-term care insurance) not only in medicine but also in dentistry.
In this paper, we discuss the current situation and issues of integrated care in Tokushima Prefecture, and discuss the future state in relation to the study of the Koyadaira district in Mima City, which is an area of disappearance.

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