ヒト骨髄間質細胞の造血支持能に関する研究
1994 年 106 巻 7-8 号 p. 837-846
詳細
1994 年 106 巻 7-8 号 p. 837-846
A bone marrow coculturing system developed by M. Y. Gordon was modified for easier evaluation of the function of bone marrow stromal cells. In the method agar was not used, and cells were dried and stained directly on a 35-mm petri dish after culture. The sustaining capacity of hemopoiesis by human stromal cells in bone marrow obtained by this method was compared between eight young and five elderly healthy volunteers. Hemopoietic progenitor cells were obtained from young volunteers and cultures were prepared for seven days.
There appeared 86±28 colonies from 105 non-adherent bone marrow cells on the sheet of stromal cells from the young volunteers and 89±27 colonies from the elderly. The colonies were classified into two types according to cytochemical appearance through peroxidase staining, but all of them proved to be composed of myeloid cells by enzyme-labeled antibody staining.
Colonies were identified easily and accurately by the newly modified coculturing method. No difference in sustaining capacity of hemopoiesis by human stromal cells in bone marrow was found between the young and the elderly using this system.
