超生体染色所見から見た血球の細胞膜透過性に関する研究

第1編 各種色素の濾紙電気泳動と,それ等色素の正常家兎白血球,網赤血球への超生体染色所見

抄録

Supravital staining of granulocyte, lymphocyte and reticulocyte was carried out with a series of dyes including both acidic and basic ones. On each dye the affinity to cells was observed in those media which pH Were changed serially from 1 to 13 using McIlvaine's or Sörensen's buffer solution. On the other hand the increasing or decreasing effect of hydrogen ion concentration of media on the charges of dyes was observed electrophoretically. In general, strongly charged dyes, both positive and negative, failed to invade into cells in the whole range of pH, while the Weakly charged dyes showed a positive stain in a certain region of pH, i. e. the acidic dyes in the acidic media and basic dyes in the basic media, showing the accelerated permeability of dyes to cells in their fairly discharged states. However, some dyes proved to be inpermeable even in their weakly charged states. This inpermeability was attributed to the large molecular weight of the dyes or to the sparing solubility to water, alcohol, ether and ether-alcohol.