超生体染色色素の固定に関する研究

第4編 各種血液細胞の観察所見

抄録

By the methods reported in the previous papers the auther observed the supravitally stained granules on the nucleus stained with hematoxylin. As the results it has been revealed that in lymphocytes, monocytes, myelocytic cells and Yoshida sarcoma cells, Janus green B stains mitochondria selectively but not the other components. The irregular forms of mitochondria stained with Janus green B can be recongnized frequently. This seems to be the result of the structural demolition caused by the staining itself. But in some cells the number of mitochondria which are stained by this dye, is rather small compared with those found under the phase contrast or the electron-microscopes. Then it is assumed that Janus green B stains mitochondria selectively but not all of them. In general neutral red seems not to stain Golgi apparatus selectively, and neutral red granules are found to be irregular, both in their distribution in cytoplasma and in their size, all the granules being almost round in shape. But in small cells like monocytes, sometimes in lymphocytes the granules tend to gather in the area of Golgi zone. This thendency is recongnized especially markedly in the cells from the lower animals In the rosette formation of the neutral red Yoshida sarcoma cells have almost the same picture as that of monocytes but their mitochondria stainable with Janus green B are larger in number compared with those of monocytes. Brilliant crecyl blue and Nile blue seem also to stain mitochondria, but some other components like endoplasmic reticulum, neutrophilic granules and others in leucocytes, which can be stained by these dyes as the stained granules are very large in number and show marked variety in their sizes.