異種臓器抗体に関する研究
第1編 犬胃及び十二指腸粘膜抽出抗原による家兎感作時の生体反応について
1959 年 71 巻 12-2 号 p. 8519-8536
詳細
1959 年 71 巻 12-2 号 p. 8519-8536
The significance of vital reaction was immunochemically observed on the sensitization of rabbit by the heterogeneous organic (canine stomach and duodenum) antigen. And the results were as follows.
1. On the sensitization of rabbit by the extracted antigen of canine stomach and duodenum with physiological salt solution, the body weight of rabbit showed a little decrease at the 1-2 week, but it gradually increased or it did not show remarkable change after then. In the blood picture, the erythrocyte count and hemoglobin value showed a little decrease around the 2-5th week after the sensitization, but the leukocyte count increased since the 1-2nd week after the sensitization and it repeatedly decreased after the 5-6th week and returned to the normal count. In the classification of leukocyte, slight neutrocytosis was seen in some cases at the first, but lymphcytosis was seen since the 4-6th week.
2. On the sensitization of rabbit by the extracted antigen of canine stomach and duodenum with pyhsiological salt solution, the production of antibody corresponding to the antigen was certified. And it became clear that the organic antibody produced by the attempt of cross-reaction with the extracted antigen of other canine abdominal organs with physiological salt solution relatively had the organic specifity in vitro.
3. Comparing the antigen of lipid, phospholipid, polysaccharide and protein etc extracted from the above described organic antigen with their antibodies' reactions to them, no remarkable difference among those antigens was observed.
4. No significant change of the complement titer was observed on the sensitization of rabbit by the extracted antigen of canine stomach and duodenum with physiological salt solution.
5. As for the hitological changes on the sensitiztion of rabbit by the extracted antigen of canine stomach and duodenum with physiological salt solution, no remarkable changes were observed on the gastric and duodenal mucosa corresponding to the antigen, and the hypertrophy of splenic follicle, multiplication of reticular tissure, hypertrophy and multiplication etc., i.e. pathologic findings of the reticuloendothelial system, were observed.
