Abstract
By carrying out vital observations on serous cells of ascites in normal persons (nonpathologic) and on tumor cells in the ascites of three patients with coelothelioma mainly by means of the simple culture method devised in our department, the author compared the results of the two observations, and obtained the following results.
1. In the smeared and fixed specimens coelothelioma cells show clearly malignant traits, but on examining them more scrutinously the transition from serous cells can be recognized.
2. In the tissue culture the nuclear membrane and cytoplasmic granules all indicate that serous cells are being transformed into coelothelioma cells.
3. In the vital staining the manner of distribution of cytoplasmic granules stained with Janus green also indicates the transformation of both cells.
4. The carbon-particle phagocytosis in both cells prove to be negative.
5. When these cells are prepared by compression method and observed under a phase-contrast microscope, from their nuclear membrane, increased nucleo-cytoplasmic ratio, and mitochondria distribution it can be understood that there exists a trasitional type of cells between both cells.
6. In T. P. T. reaction, the tumor characteristic of coelothelioma cells is found to be weak.
7. In the phase-contrast microscopic observation conducted on the tissue culture of both cells in suspension, more transitional type of both cells can be recognized than in the case of compression method.
From these observations it has been confirmed that coelothelioma cells are fundamentally derived from serous cells, and that these tumor cells are generally flat, possessing various pseudopodia which can move actively and they have complicated pecuiiar large vacuoles, and that these can be construed as three great charateristic traits of these tumor cells.
