1968 年 80 巻 5-6 号 p. 619-623
For the spectrophotometry a Hitachi Perkin-Elmer 139 type spectrophotometer with a constant temperature cell chamber and autorecorder was used.
The measurements were conducted under the following conditions (final concentration of H2O2 0.0125 Mol in 0.01 Mol phosphate buffer, pH 6.8), which showed the identical conditions with a exception of H2O2 being 2.5 times the concentration employed in the titration method.
Both assays were carried out at 37°C.
The results by ultraviolet spectrophotometric method (UV method) were compared with those obtained by titration; the values obtained by UV method were about 90% of those by titration, and proved that each values showed a good correlation. Therefore this method will sufficiently serve for the measurement of human blood catalase activity or for the screening of hypocatalasemia.