1974 Volume 86 Issue 7-8 Pages 333-350
1. Before applying the fluorescence microscopic method with Coriphosphine O (CO) to the staining of gastric mucosal tissues. the type of solvent, PH and temperature for making most of the physicochemical properties of Coriphosphine O pigment were -examined, and the opitimal staining conditions were established.
2. The CO fluorescence microscopic method was found to be capable of well indicating the tissue structure of the gastric mucosa histomorphologically. The method also enabled the visual identification of the individual cells, and could be used for histopathological diagnosis.
3. The fluorescence microscopic method by use of CO fluorescent pigment is characterized by specific binding of the pigment with nucleic acid, as has been demonstrated by digestion with DNAse and RNAse.
Dilutions in various concentrations of CO pigment with McIlvaine buffer, PH 5.4, were, when irradiated with ultraviolet rays in the vicinity of the wavelength 405μm, found to show a spectrum of color changes according to the pigment concentrations. These color changes were in agreement with those of the gastric mucosal epithelial cell eytoplasm in various gastric diseases under application of the CO fluorescence microscopic method.
4. By comparing the color changes of gastric mucosal epithelial cell cytoplasm with concentrations in vitro, the average CO concentration of pathologic mucosal epithelial cell cytoplasm in various stomach diseases and RI (RNA Index) were calculated and comparatively stadied.
5. The comparative study of the average CO concentration and RI, i.e., the fluorescent color of the epithelial cell cytoplasm alone was found to have littel usefulness for clinical purposes. This was pointed out to be an important matter in clinical cytodiagnosis.
6. As far as the RNA level within the epithlial cell cytoplasm was concerned, the average CO concentration and RI. could be used as a yardstick of RNA level in the cell, only under a simple morphological provision that “the epithelial cell cytoplasm is stained diffusely.”
7. As compared with normal mucosa, an increase in cell RNA was observed in the mucoosa of cancerous tissues of early as well as advanced cancer, in the rostral noncancerous stump tissue of advanced cancer, in a part of the noncancerous tissue adjacent to cancer, and in the regenerated epithlium. The clinical significance of these findings was discussed.
8. The cells with remarkable mucous retention, i.e., goblet cells in the mucosal epithelium with intestinal metaplasia, signet-ring cells within mucus-producing cancerous tissues, and the mucous portion of lacunar epithlial cell cytoplasm in adenomatous polyps, were not stained.
