二価鉄誘導脂質過酸化反応に関する研究

第一編 二価鉄誘導脂質過酸化反応溶液中の二価鉄の定量

抄録

The 2-nitroso-(N-propyl-N-sulfopropylamino)phenol(Nitroso-PSAP), one of the watersoluble derivatives of nitrosoaminophenol, is a very sensitive reagent for the spectrophotometric determination of Fe (II). Using Nitroso-PSAP reagent, the author investigated the conditions for determining Fe (II) quantitatively and selectively the system of Fe (II)-induced lipid peroxidation, and obtained the following results.
1) Fe (II) could be measured quantitatively without reductant. The Fe (II) complex had a molar absorptivity of 4.5×10⁴ dm³ mol^-1 cm^-1 at 756 nm.
2) Fe (II) could be measured quantitatively in the TCA solution removed from the reaction medium for lipid peroxidation. The Fe (II) complex had a molar absorptivity of 3.14×10⁴ dm³ mol^-1 at 756 nm.
3) Fe (II) could be measured quantitatively and selectivily under conditions involving a mixture of Fe (II).