ヒト肺の肥満細胞に関する研究
第1編 ヒト肺肥満細胞の分離精製に関する検討
1986 年 98 巻 5-6 号 p. 475-485
詳細
1986 年 98 巻 5-6 号 p. 475-485
In order to clarify the pathophysiology of allergic disorders, it is necessary to study the reactivity of mast cells. Recently, evidence that mast cells have specificities in species and organs has been reported. For use in an investigation of bronchial asthma, I developed a suitable technique for the purification of mast cells using normal lung tissue from patients with lung cancer. The purification steps were: (1) dispersion of lung fragments into a single cell suspension with an enzyme mixture (pronase-chymopapain and collagenase-elastase), (2) centrifugation using a Percoll continuous gradient, and (3) exclusion of adherent cells. As a result, 2.1±1.1×105 mast cells per gram of lung tissue in purities of 38.8±7.2% were obtained. Isolated mast cells proved functionally and morphologically stable by the following findings. The cells showed: (1) dye exclusion exceeding 95%, (2) a histamine content of 4.3±0.6pg/mast cell, (3) a histamine release of 47.9±11.2% induced by calcium ionophore A 23187, and (4) almost no damage on scanning electron microscopic observation. These results indicate that the present technique for the purification of human lung mast cells is comparatively simple and useful. The isolated mast cells can be applied to pathogenetic and therapeutic studies of bronchial asthma.
