Abstract
We investigated the bacteriolytic activity of alkaline protease BYA, a detergent protease, from Bacillus sp. Y. Protease BYA effectively lysed gram-negative bacteria and some gram-positive bacteria like Staphylococcus aureus and Staphylococcus epidermidis. The optimum pH of protease BYA was 8-9, and it was most active at 60°C, a temperature at which Deozyme, a commercial bacteriolytic enzyme, had lost most of its activity. The bacteriolytic activity and protease activities of protease BYA would be useful for removing bacteria from worn garments and production facilities.