Journal of Oleo Science
Online ISSN : 1347-3352
Print ISSN : 1345-8957
ISSN-L : 1345-8957
Oils & Fats
Large Scale Production of Liquid Wax Ester by Immobilized Lipase
Salina Mat RADZIMahiran BASRIAbu Bakar SALLEHArbakariya ARIFFRosfarizan MOHAMMADMohd. Basyaruddin Abd RAHMANRaja Noor Zaliha Raja Abd. RAHMAN
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2005 Volume 54 Issue 4 Pages 203-209


Oleyl oleate, a liquid wax ester was synthesized by an immobilized Candida antartica lipase B (Novozym 435) as biocatalyst using oleic acid and oleyl alcohol. The effects of various reaction parameters were optimized to obtain a high yield of liquid wax esters. Investigation in large scale production was performed in batch mode of stirred tank reactor (STR) with one multi-bladed impeller. The optimum condition to produce liquid wax ester was, reaction time (RT); 30 min, temperature (T); 50 °C, amount of enzyme (E); 90 g (900,000 PLU), agitation speed (A) of 400 rpm, number of impeller tip (N) of 2 and molar ratio of oleyl alcohol to oleic acid (M); 2:1. Analysis of the yield showed that at optimum condition, >90% liquid wax esters were produced. The stability of Novozym 435 showed at high percentage (>80%) up to 4 cycles.

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© 2005 by Japan Oil Chemists' Society
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