Formation of W/O Microemulsion Based on Natural Glycolipid Biosurfactant, Mannosylerythritol Lipid-A

Wannasiri Worakitkanchanakul; Tomohiro Imura; Tomotake Morita; Tokuma Fukuoka; Hideki Sakai; Masahiko Abe; Ratana Rujiravanit; Sumaeth Chavadej; Dai Kitamoto

doi:10.5650/jos.57.55

Rapid Paper

Formation of W/O Microemulsion Based on Natural Glycolipid Biosurfactant, Mannosylerythritol Lipid-A

Wannasiri Worakitkanchanakul, Tomohiro Imura, Tomotake Morita, Tokuma Fukuoka, Hideki Sakai, Masahiko Abe, Ratana Rujiravanit, Sumaeth Chavadej, Dai Kitamoto

Author information

Wannasiri Worakitkanchanakul
Faculty of Science and Technology, Tokyo University of Science
Tomohiro Imura
Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST)
Tomotake Morita
Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST)
Tokuma Fukuoka
Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST)
Hideki Sakai
Faculty of Science and Technology, Tokyo University of Science
Institute of Colloid and Interfacial Science, Tokyo University of Science
Masahiko Abe
Faculty of Science and Technology, Tokyo University of Science
Institute of Colloid and Interfacial Science, Tokyo University of Science
Ratana Rujiravanit
The Petroleum and Petrochemical College, Chulalongkorn University
Sumaeth Chavadej
The Petroleum and Petrochemical College, Chulalongkorn University
Dai Kitamoto
Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST)

Keywords: glycolipid biosurfactant, mannosylerythritol lipid-A, W/O microemulsion, freeze fracture electron microscopy (FF-EM), dynamic light scattering (DLS)

JOURNAL FREE ACCESS

2008 Volume 57 Issue 1 Pages 55-59

DOI https://doi.org/10.5650/jos.57.55

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Abstract

Mannosylerythritol lipid-A (MEL-A) is a glycolipid biosurfactant abundantly produced from soybean oil by microorganisms at a yield of up to 100 g/L. In this study, the formation of water-in-oil (W/O) microemulsion based on the single component of MEL-A was confirmed using dynamic light scattering (DLS) and freeze fracture electron microscopy (FF-EM). DLS and FF-EM measurements revealed that the diameter of the microemulsion increases with an increase in water-to-surfactant mole ratio (W₀) ranging from 20 to 60 nm, and the maximum W₀ value was found to be 20, which is as high as that of soybean lecithin. Glycolipid biosurfactant has a great potential for the formation of W/O microemulsion without using any cosurfactants.

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