Journal of Oleo Science
Online ISSN : 1347-3352
Print ISSN : 1345-8957
ISSN-L : 1345-8957
Oils and Fats
Chromatographic Quantitation of Some Bioactive Minor Components in Oils of Wheat Germ and Grape Seeds Produced as By-Products
Minar Mahmoud M. HassaneinAdel Gabr Abedel-Razek
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2009 Volume 58 Issue 5 Pages 227-233


The objective of this work was to determine some beneficial and bioactive minor lipid components in wheat germ and grape seeds as a result of milling industry of wheat and pressing of grapes in wineries. Sterylglycosides (SG’s) were isolated and fractionate into free and acylated SG’s by TLC and were determine as their1-anthroylnitriles (1-AN) by HPLC. Moreover, 4-desmethylsterols were isolated, derivatized into their trimethyl silyl derivatives and analyzed by GLC. Tocopherols and tocotrienols were directly analyzed by HPLC. In addition, fatty acids composition by GLC was accomplished. The results were compared to three conventional edible oils, namely, corn, sunflower and cottonseed. It was found that the wheat germ oil (WGO) and grape seed oil (GSO) contained reasonable amounts of whole sterols. Sterylglycosides fraction (SG), which have not been evaluated, it was found that the two by-products contained high amounts of SG’s and they were rich in free and acylated campe/stigma SG as well as free and acylated β-sito SG. Total tocopherols and tocotrienols components were found in very high amounts in WGO (1300 ppm) and GSO (380 ppm). It is noteworthy to mention that GSO contained significant amounts of alpha- and gamma-tocotrienols which prevent cardiovascular diseases and contained reasonable amounts of alpha- and gamma-tocopherols. On the other side, it was found that WG and GS oils were enrich in linoleic acid (omega-6), while linolenic acid (omega-3) was present in higher quantity in WGO.

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© 2009 by Japan Oil Chemists' Society
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