Journal of Oleo Science
Online ISSN : 1347-3352
Print ISSN : 1345-8957
ISSN-L : 1345-8957
Biochemistry and Biotechnology
Effects of Cold Shock Treatment on Total Lipid Content and Fatty Acid Composition of Aurantiochytrium limacinum strain mh0186
Yousuke TaokaNaoki NaganoYuji OkitaHitoshi IzumidaShinichi SugimotoMasahiro Hayashi
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JOURNAL FREE ACCESS

2011 Volume 60 Issue 5 Pages 217-220

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Abstract

To examine the effect of cold shock treatment on the fatty acid composition of Aurantiochytrium limacinum strain mh0186, a marine thraustochytrid, we cultivated this strain at 28°C for 72 h with shaking and stored the obtained biomass at 10°C for 72 h. A growth experiment was carried out for comparison, wherein strain mh0186 was grown at 10 and 15°C for 72 h with shaking, and it was found that the unsaturation of fatty acids was accelerated relative to that at 28°C. In the cold shock experiment, the total lipid content significantly increased during storage at 10°C for 72 h. Overall, the percentage of unsaturated fatty acids such as docosahexaenoic acid was almost stable while that of n-6 docosapentaenoic acid decreased slightly, but significantly, relative to that in the growth experiment.

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© 2011 by Japan Oil Chemists' Society
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