Conjugated Linoleic Acids Inhibit Hypoxia Inducible Factor-1α Stabilization under Hypoxic Condition in Human Hepatocellular Carcinoma Cells

Masao Yamasaki; Taku Nagatomo; Tetsuya Matsuyama; Yuri Ikeho; Emiko Kato; Kei Nishiyama; Yoichi Sakakibara; Masahito Suiko; Kazuo Nishiyama

doi:10.5650/jos.61.491

Biochemistry and Biotechnology

Conjugated Linoleic Acids Inhibit Hypoxia Inducible Factor-1α Stabilization under Hypoxic Condition in Human Hepatocellular Carcinoma Cells

Masao Yamasaki, Taku Nagatomo, Tetsuya Matsuyama, Yuri Ikeho, Emiko Kato, Kei Nishiyama, Yoichi Sakakibara, Masahito Suiko, Kazuo Nishiyama

Author information

Masao Yamasaki
Division of Food Science and Nutrition, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
Taku Nagatomo
Division of Food Science and Nutrition, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
Tetsuya Matsuyama
Division of Food Science and Nutrition, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
Yuri Ikeho
Division of Food Science and Nutrition, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
Emiko Kato
Division of Food Science and Nutrition, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
Kei Nishiyama
Division of Food Science and Nutrition, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
Yoichi Sakakibara
Division of Food Science and Nutrition, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
Masahito Suiko
Division of Food Science and Nutrition, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki
Kazuo Nishiyama
Division of Food Science and Nutrition, Department of Biochemistry and Applied Biosciences, Faculty of Agriculture, University of Miyazaki

Keywords: conjugated linoleic acid, hypoxia inducible factor, apoptosis, hepatocellular carcinoma

JOURNAL FREE ACCESS

2012 Volume 61 Issue 9 Pages 491-496

DOI https://doi.org/10.5650/jos.61.491

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Abstract

The anti-cancer effects of various fatty acids are drawing a lot of attention. To determine whether different fatty acids affect the hypoxic response of liver cancer cells, we examined the effects of various fatty acids on the stabilization of the hypoxia-inducible factor (HIF)-1α protein in the HepG2 human hepatocellular carcinoma (HCC) cell line under condition containing 1% O₂. Of the fatty acids examined, only 9cis, 11trans (c9, t11)-conjugated linoleic acid (CLA) and 10trans, 12cis (t10, c12)-CLA inhibited hypoxia-induced HIF-1α stabilization. In addition, HIF-1α prolyl hydroxylase or proteasome inhibition abrogated the effects of c9, t11- and t10, c12-CLA. Moreover, c9, t11- and t10, c12-CLA significantly inhibited cell proliferation and induced apoptotic cell death under hypoxia. This is the first study showing that c9, t11- and t10, c12-CLA inhibit the hypoxic response in HCC cells.

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