A Comparison of the Lipid-lowering Effects of Four Different n-3 Highly Unsaturated Fatty Acids in HepG2 Cells

Koji Nagao; Kazusa Nakamitsu; Hiroki Ishida; Kazuaki Yoshinaga; Toshiharu Nagai; Hoyo Mizobe; Koichi Kojima; Teruyoshi Yanagita; Fumiaki Beppu; Naohiro Gotoh

doi:10.5650/jos.ess14118

Oils and Fats

A Comparison of the Lipid-lowering Effects of Four Different n-3 Highly Unsaturated Fatty Acids in HepG2 Cells

Koji Nagao, Kazusa Nakamitsu, Hiroki Ishida, Kazuaki Yoshinaga, Toshiharu Nagai, Hoyo Mizobe, Koichi Kojima, Teruyoshi Yanagita, Fumiaki Beppu, Naohiro Gotoh

Author information

Koji Nagao
Department of Applied Biochemistry and Food Science, Saga University
Kazusa Nakamitsu
Department of Applied Biochemistry and Food Science, Saga University
Hiroki Ishida
Department of Food Science and Technology, Tokyo University of Marine Science and Technology
Kazuaki Yoshinaga
Tsukishima Foods Industry Co. Ltd.
Toshiharu Nagai
Tsukishima Foods Industry Co. Ltd.
Hoyo Mizobe
Tsukishima Foods Industry Co. Ltd.
Koichi Kojima
Tsukishima Foods Industry Co. Ltd.
Teruyoshi Yanagita
Department of Health and Nutrition Science, Nishikyushu University
Fumiaki Beppu
Department of Food Science and Technology, Tokyo University of Marine Science and Technology
Naohiro Gotoh
Department of Food Science and Technology, Tokyo University of Marine Science and Technology

Keywords: docosahexaenoic acid, docosapentaenoic acid, eicosapentaenoic acid, HepG2 cell, lipid metabolism, n-3 highly unsaturated fatty acid, tetracosahexaenoic acid

JOURNAL FREE ACCESS

2014 Volume 63 Issue 10 Pages 979-985

DOI https://doi.org/10.5650/jos.ess14118

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Abstract

The effects on lipid metabolism of four different n-3 highly unsaturated fatty acids (n-3HUFA) including eicosapentaenoic acid (EPA, 20:5n-3), docosapentaenoic acid (DPA, 22:5n-3), docosahexaenoic acid (DHA, 22:6n-3), and tetracosahexaenoic acid (THA, 24:6n-3) were compared in the HepG2 cell model. None of the n-3HUFAs affected the viability of the cells. THA exerted the strongest suppression on the synthesis of triacylglycerol and cholesteryl ester (ChE), and the order of the strength of suppression was found to be THA > DHA > DPA > EPA. The mRNA level of fatty acid synthase was suppressed by the n-3HUFAs and the order of the strength of suppression by n-3HUFAs was the same in both triacylglycerol and ChE synthesis. These findings support previous animal test results using EPA, DPA, and DHA. In conclusion, both the number of carbon atoms and double bonds in an n-3HUFA structure has an effect on lipid metabolism in HepG2 cells.

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